Thursday, May 31, 2012

Phototropism, Geotropism….. Scentropism?


Tropisms are directional growth responses to external stimuli. Common tropisms include phototropism and geotropism—both of which we discussed in biology class. Phototropism is positive for plant stems and negative for roots—that is, the stem will lean towards sunlight as the plant grows while the roots will grow away from the light. The opposite is true for geotropism—growth in response to gravity—as the stems grow counter to the force of gravity, and the roots grow in the direction gravity is acting, which is down. These tropisms seem reasonable enough. Plants use sunlight for photosynthesis so it makes sense that they would grow towards it in order to maximize the amount of sunlight they are exposed to. But what of parasitic plants without the ability to photosynthesize?

Cuscuta pentagona—an orange vine with small white flowerslacks chlorophyll and therefore is unable to convert light energy into chemical energy and make its own food. It would not benefit from phototropism, which leads plants to the light energy source they need to produce sustenance. Sure, the Cuscuta grows its roots into the dirt and its vines upward, like other plants. However, it attains the nutrients it needs by attaching itself to a host plant and sending “microprojections” into the tomato’s phloem - through which the plant transports sugars (mostly sucrose, but also glucose), amino acids, plant hormones, and mRNA—to siphon off the host plant’s photosynthesized sugar supplies. Without a host to live off of, a young Cuscuta plant will inevitably die. To prevent this from happening, the Cuscuta has a plant-detecting mechanism by which it locates the target host plant it will attack through smell.

Cuscuta pentagona commonly latches onto tomato plants. As a Cuscuta pentagona seedling grows, it probes its surroundings, growing and rotating its shoot tip until it finds a tomato (or other) plant in its vicinity, then wraps around this new host’s stem in order to have access to the plant’s phloem. Consuelo De Moraes hypothesized that the Cuscuta or dodder plant detected its host through “chemical signaling.” She noticed that, despite variable conditions—light or shade, in the presence of other plants (specifically, wheat), empty pots, or  pots with fake plants—dodder vines always grew towards the tomato plants! To test her hypothesis, she synthesized a tomato perfume, or “eau de tomato”, with extracts from the stem of a tomato plant. She then soaked cotton swabs with this perfume and placed them on sticks in a pot next to the dodder. As she hypothesized, the dodder indeed grew towards the cotton that gave off the scent of a tomato plant. Dodders grow towards tomato plants because tomato plants contain beta-myrcene, in addition to two other chemicals. All three of these chemicals are easily turned into gases and give off odors which attract dodders.

This is not the only case in which non-tactile plant communication occurs. University of Washington scientists David Rhoades and Gordon Orians, observed that willow trees neighboring those that were plagued by caterpillars were less likely to be attacked by caterpillars. Upon investigation, Rhoades found that the leaves of trees  found next to, but not touching, the infested willows contained phenolic and tannin—chemicals that repel the insects which feed on them. These chemicals were absent in healthy willows which were not surrounded by caterpillar-plagued willows. Since neither the roots nor branches of the damaged and healthy trees had contact with each other, Rhoades proposed that, via pheromones, the infested trees sent a warning message to healthy trees so that they could defend themselves against the impending insect attack.

Plants aren’t as altruistic as they seem, however, as Martin Heil, a Mexican scientist, found. Heil posited that the infected plants didn’t intend on warning their neighbors at allthe pheromones released by damaged leaves were directed at the remaining healthy leaves of the infested plant. Neighboring plants simply detected these pheromones and reacted to protect themselves. To test his hypothesis, Heil isolated damaged leaves in a sealed plastic bag to determine whether this disrupts pheromone communication. As he had suspected, the damaged leaves were unable to transmit their warning message to healthy leaves on the same plant, and these healthy leaves remained the same. Heil concluded that the chemicals released by damaged leaves are necessary for the rest of the plant to defend itself from further attacks.

Of course, plants don’t smell in the same way that we dowith nerves that send signals to our brain and tell us of what scents are around usbut they are most definitely capable of detecting and responding to chemicals in the air around them. This makes a lot of sense. We all know that plants, flowers especially, give off odors and aromas which attract pollinators and vectors, like nocturnal animals, for seed transport. But, just as humans speak and can hear, plants give off odors and can detect scents.

I found this really eye-opening and intriguing. It shows that we can still discover new things about even seemingly simple organisms with which we are familiar. Although we don’t have enough information to conclude definitively the nature of pheromone communication, Heil’s experiment doesn’t rule out the existence of a warning system. The chemicals released by damaged leaves may be necessary for the survival of the healthy leaves, but this fact alone doesn’t preclude the intention of the infected plant to warn neighboring plants of its plight. It would be interesting to explore the details of pheromone communication, how it varies, if at all, among different plant species. Pheromones could be the key to giving us a better understanding of plant communication. Another thing I found ironic is that humans, although thought to be one of the most complex organisms with conscious thought, aren’t capable of understanding the pheromones we release, whereas plants can. Maybe we aren’t as superior to other organisms as some might think.

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